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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital function in endorsing mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually described. Two added splice variants ended up described but thorough nucleotide sequence info isn't still obtainable.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide typically has the epitope recognized through the antibody.
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This information outlines even more doable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...
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This information outlines additional probable optimization of cellular and immunoassay parameters to be certain the best possible success are...
Antibodies certain to the blocking peptide no longer bind for the epitope to the target protein. By comparing the staining from your blocked antibody vs . the antibody by itself, one can see which staining is distinct.
Concentrate on Information Cyclin E belongs for the remarkably conserved cyclin family, whose members are characterized by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation designs which contribute on the temporal coordination of every mitotic celebration. Cyclin E sorts a fancy with and features as being a regulatory subunit of CDK2, whose activity is necessary for website cell cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells progress via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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